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Contraindications include history of hypersensitivity and severe hepatic impairment

Pups were anesthetized with isoflurane 12% mixed with warm compressed air/oxygen for the MRS scans, then placed on a MR compatible, heated animal bed (temperature maintained at 38.0 0.2 C) connected to an animal monitoring unit (SA Instruments, Inc., Stony Brook, NY), which recorded respirations, pulse oximetry, and core temperature

For HFD feeding, the mice were maintained on a HFD (D12492) (60.0 % kcal from fat) (Research Diets Inc., New Brunswick, NJ, USA) starting at 8 weeks of age unless stated otherwise

Messersmith, W

The raw files were searched against a mouse database with 59,423 entries downloaded from Swiss-Prot, TrEMBL, and UCSC databases